3EE3
Crystal structure of Acanthamoeba polyphaga mimivirus nucleoside diphosphate kinase complexed with CDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-07-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.064 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.419, 154.419, 187.039 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.19635 |
| Rwork | 0.194 |
| R-free | 0.23347 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.542 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.300 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 45481 | |
| <I/σ(I)> | 6 | 1.7 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 40 to 44% MPD, 0.1M MOPS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
