3EDH
Crystal structure of bone morphogenetic protein 1 protease domain in complex with partially bound DMSO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.499, 59.093, 69.451 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.990 - 1.250 |
| R-factor | 0.142 |
| Rwork | 0.140 |
| R-free | 0.17200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3edi |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.578 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.090 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.083 | 0.394 |
| Number of reflections | 60273 | |
| <I/σ(I)> | 15.5 | 3.66 |
| Completeness [%] | 97.9 | 96.1 |
| Redundancy | 6 | 5.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | 5% DMSO, pH 7.5, vapor diffusion, temperature 277K |
