3ECB
Crystal structure of mouse H-2Dd in complex with peptide P18-I10 derived from human immunodeficiency virus envelope glycoprotein 120
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Collection date | 2008-07-10 |
Wavelength(s) | 1.0809 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.727, 89.452, 109.705 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.798 - 1.698 |
R-factor | 0.1839 |
Rwork | 0.182 |
R-free | 0.22730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ddh |
RMSD bond length | 0.005 |
RMSD bond angle | 1.017 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.060 | 0.440 |
Number of reflections | 51683 | |
<I/σ(I)> | 3.7 | |
Redundancy | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 16% PEG 3350, 0.2M Magnesium formate, , pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |