3EBO
Glycogen Phosphorylase b/Chrysin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2008-06-13 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.038 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.834, 128.834, 116.635 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.230 - 1.900 |
R-factor | 0.19573 |
Rwork | 0.194 |
R-free | 0.21957 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1hlf |
RMSD bond length | 0.007 |
RMSD bond angle | 1.012 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.290 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 71586 | |
<I/σ(I)> | 15.6 | 4.4 |
Completeness [%] | 97.3 | 96.7 |
Redundancy | 5.3 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 286 | BES, EDTA, pH 6.7, SMALL TUBES, temperature 286K |