3EAW
Replacement of Val3 in Human Thymidylate Synthase Affects Its Kinetic Properties and Intracellular Stability
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Detector technology | CCD |
| Collection date | 2008-04-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 95.704, 95.704, 81.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.860 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.26800 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.010 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.061 | 0.392 |
| Number of reflections | 33751 | |
| <I/σ(I)> | 35.545 | |
| Completeness [%] | 91.6 | 57.5 |
| Redundancy | 5.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 20-25%ammonium sulfate, 20mM BME, 0.1M Tris PH8.5 , VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
