3EAM
An open-pore structure of a bacterial pentameric ligand-gated ion channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.320, 133.640, 160.180 |
| Unit cell angles | 90.00, 102.94, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.900 |
| R-factor | 0.20046 |
| Rwork | 0.199 |
| R-free | 0.23110 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.034 |
| Refinement software | REFMAC (5.4.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.101 | 0.626 |
| Number of reflections | 83260 | |
| <I/σ(I)> | 11.34 | 2.05 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG 4000, NH4SCN, NaAc, pH 4.6, vapor diffusion, hanging drop, temperature 298K |
