3EAK
NbBCII10 humanized (FGLA mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.909 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 75.585, 70.750, 50.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.950 |
Rwork | 0.215 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zmy |
RMSD bond length | 0.006 |
RMSD bond angle | 1.370 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.196 | 0.607 |
Number of reflections | 20171 | |
<I/σ(I)> | 9.99 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.4 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM Na-Hepes pH 7.5, 2% PEG 400, 2M NH4(SO4)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |