3EAH

Structure of inhibited human eNOS oxygenase domain

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	71.090, 90.150, 156.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.440
Rwork	0.213
R-free	0.25800
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.440
Number of reflections	36307

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K