3E9V
Crystal structure of human B-cell Translocation Gene 2 (BTG2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-10 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.535, 41.023, 68.652 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.220 - 1.700 |
R-factor | 0.213 |
Rwork | 0.211 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2d5r |
RMSD bond length | 0.012 |
RMSD bond angle | 1.431 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.21) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.030 | 41.030 | 1.790 |
High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
Rmerge | 0.071 | 0.046 | 0.457 |
Total number of observations | 5388 | 23988 | |
Number of reflections | 12646 | ||
<I/σ(I)> | 6.69 | 12.7 | 1.5 |
Completeness [%] | 98.7 | 97.3 | 97.6 |
Redundancy | 13.8 | 11.7 | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 100mM Bis-Tris pH 6.5, 0.2M Magnesium Chloride, 25% PEG 3350, Vapor diffusion, sitting drop, temperature 294K |