3E73
Crystal Structure of Human LanCL1 complexed with GSH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Collection date | 2007-04-10 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 194.100, 194.101, 243.554 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.330 - 2.800 |
| R-factor | 0.23272 |
| Rwork | 0.231 |
| R-free | 0.26244 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e6u |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.330 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.330 | 2.873 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 59222 | |
| Completeness [%] | 88.4 | 64.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 1.5M ammonium formate, 0.1M bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
