3E6V

X-ray structure of human arginase I-D183N mutant: the complex with ABH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Wavelength(s)	0.89
Spacegroup name	P 3
Unit cell lengths	91.036, 91.036, 69.773
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.720
Rwork	0.219
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zav
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.820
High resolution limit [Å]	1.720	1.720
Rmerge	0.058	0.400
Number of reflections	66499
<I/σ(I)>	17.5	2
Completeness [%]	97.0	96.7
Redundancy	2.2	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1