3E3N
The Glycogen phosphorylase b R state- AMP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2002-11-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 119.019, 188.079, 175.913 |
| Unit cell angles | 90.00, 108.92, 90.00 |
Refinement procedure
| Resolution | 29.790 - 2.700 |
| R-factor | 0.19517 |
| Rwork | 0.192 |
| R-free | 0.25609 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1abb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.297 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.850 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 189160 | |
| <I/σ(I)> | 6.2 | 1.67 |
| Completeness [%] | 94.3 | 92.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.5 | 289 | 1.2-1.4 M ammonium sulphate, pH 7.5, MICRODIALYSIS, temperature 289K |
