3E30
Protein farnesyltransferase complexed with FPP and ethylene diamine inhibitor 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 173.307, 173.307, 70.147 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.720 - 2.450 |
R-factor | 0.18621 |
Rwork | 0.184 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.096 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.720 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 41034 | |
Completeness [%] | 97.6 | 76.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 290 | ammonium acetate pH 5.7, 12% PEG 8000, DTT, VAPOR DIFFUSION, HANGING DROP, temperature 290K |