3E2V
Crystal structure of an uncharacterized amidohydrolase from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.532, 55.007, 115.260 |
| Unit cell angles | 90.00, 95.85, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.500 |
| R-factor | 0.174 |
| Rwork | 0.173 |
| R-free | 0.19900 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.238 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 114.708 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.095 | 0.413 |
| Total number of observations | 116098 | |
| Number of reflections | 123244 | |
| <I/σ(I)> | 13.7 | 3.6 |
| Completeness [%] | 98.7 | 97.3 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 294 | 100mM Tris HCl pH 9, 32% PEG 4K, 200mM magnesium chloride hexahydrate, pH 9.0, Vapor diffusion, temperature 294K |
