3E0G
Structure of the Leukemia Inhibitory Factor Receptor (LIF-R) domains D1-D5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 140 |
| Detector technology | CCD |
| Wavelength(s) | 0.97945 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.008, 143.169, 80.362 |
| Unit cell angles | 90.00, 110.38, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.100 |
| R-factor | 0.25476 |
| Rwork | 0.252 |
| R-free | 0.30862 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | mouse LIF-R |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.363 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.098 | 0.611 |
| Number of reflections | 16991 | |
| <I/σ(I)> | 10.8 | 2 |
| Completeness [%] | 98.2 | 98.9 |
| Redundancy | 3.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 0.1 M Sodium Citrate pH 5.0, 0.1 M Lithium Sulfate, 8% PEG-3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
