3DZ8
Crystal structure of human Rab3B GTPase bound with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E DW |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-05-13 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 56.969, 50.875, 73.553 |
Unit cell angles | 90.00, 110.91, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.213 |
Rwork | 0.211 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gf9 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.381 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.061 | 0.030 | 0.609 |
Number of reflections | 15627 | ||
Completeness [%] | 99.7 | 100 | 97.9 |
Redundancy | 6.7 | 7.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 2M ammonium sulfate, 0.2M sodium acetate, 0.1M HEPES, 5% MPD, pH 7.5, vapor diffusion, sitting drop, temperature 291K |