3DZ2
Human AdoMetDC with 5'-[(3-aminopropyl)methylamino]-5'deoxy-8-methyladenosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9771 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.088, 50.745, 69.039 |
Unit cell angles | 90.00, 105.56, 90.00 |
Refinement procedure
Resolution | 28.480 - 1.860 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.23200 |
Starting model (for MR) | 1I7B.pdb |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.071 | 0.391 |
Number of reflections | 25965 | |
<I/σ(I)> | 14.2 | 2.2 |
Completeness [%] | 91.9 | 96.8 |
Redundancy | 2.8 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 16% PEG 8000, 100 mM Tris, 10 mM DTT, pH 8.0, vapor diffusion, hanging drop, temperature 295K |