3DYQ
human phosphodiestrase 9 (inhibited by omitting divalent cation) in complex with cGMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 103.650, 103.650, 270.041 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.19885 |
| Rwork | 0.196 |
| R-free | 0.22627 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1TBM |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.350 |
| Refinement software | REFMAC (5.3.0008) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.085 |
| Number of reflections | 65299 |
| <I/σ(I)> | 23 |
| Completeness [%] | 98.7 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
