3DYD
Human Tyrosine Aminotransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93300 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.420, 91.590, 75.570 |
Unit cell angles | 90.00, 106.18, 90.00 |
Refinement procedure
Resolution | 36.910 - 2.300 |
R-factor | 0.22158 |
Rwork | 0.219 |
R-free | 0.26088 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bw0 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.155 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.115 | 0.492 |
Number of reflections | 44821 | |
<I/σ(I)> | 10.7 | 3.3 |
Completeness [%] | 99.5 | 99.3 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 30% MPD, 0.1M Na-Acetate pH 4.6, 3mM PLP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |