3DY4
Crystal structure of yeast 20S proteasome in complex with spirolactacystin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2007-06-30 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 135.160, 301.973, 144.050 |
Unit cell angles | 90.00, 112.86, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.25600 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1ryp |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.810 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.142 | 0.592 |
Number of reflections | 254111 | |
<I/σ(I)> | 1.9 | |
Completeness [%] | 97.8 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 0.1 M MES, 10 % MPD, 20 mM MgAc2, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |