3DVN
Crystal structure of K63-specific fab Apu2.16 bound to K63-linked di-ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97945 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.705, 94.571, 97.740 |
Unit cell angles | 90.00, 107.21, 90.00 |
Refinement procedure
Resolution | 29.910 - 2.700 |
R-factor | 0.22268 |
Rwork | 0.220 |
R-free | 0.26780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.175 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 42343 | |
Completeness [%] | 99.2 | 100 |
Redundancy | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 291 | protein: 12.7 mg/mL in 10 mM Tris-HCl pH 8.0, 75 mM NaCl well: 0.2M Na Cl, 0.1 M Tris pH 8.2, 0.1 M citrate, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 291K |