3DVI
Crystal structure of kappa 1 amyloidogenic light chain variable domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-20 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5241 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 47.020, 47.020, 103.356 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 103.140 - 1.530 |
| R-factor | 0.19138 |
| Rwork | 0.189 |
| R-free | 0.25144 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q20 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.880 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.140 | 1.580 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.072 | 0.365 |
| Number of reflections | 16734 | |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 91.0 | 35.7 |
| Redundancy | 16.62 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 295 | Protein: 1.26 mM. Mother Liquor: 15% w/v polyethylene glycol 8000, 0.05 M magnesium acetate, 0.1 M sodium acetate, 0.1 M Tris buffer, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
