3DUH
Structure of Interleukin-23
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 140 |
Detector technology | CCD |
Collection date | 2008-05-04 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 116.089, 59.974, 160.365 |
Unit cell angles | 90.00, 90.46, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.23007 |
Rwork | 0.228 |
R-free | 0.26833 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | human p40 from PDB ID 1F45 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.527 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.073 | 0.556 |
Number of reflections | 49424 | |
<I/σ(I)> | 13.6 | 2.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 20% PEG3350, 0.2M potassium nitrate, 0.1M Hepes-NaOH pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |