3DSD
Crystal structure of P. furiosus Mre11-H85S bound to a branched DNA and manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Wavelength(s) | 1.1159 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.157, 88.128, 137.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.20196 |
Rwork | 0.200 |
R-free | 0.24606 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ii7 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.390 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.071 |
Number of reflections | 47737 |
<I/σ(I)> | 20.1 |
Completeness [%] | 98.5 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 40% PEG 200, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 200 | ||
2 | 1 | 1 | HEPES | ||
3 | 1 | 2 | PEG 200 | ||
4 | 1 | 2 | HEPES |