3DQB
Crystal structure of the active G-protein-coupled receptor opsin in complex with a C-terminal peptide derived from the Galpha subunit of transducin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9184 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 238.020, 238.020, 109.132 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.220 - 3.200 |
| R-factor | 0.21475 |
| Rwork | 0.213 |
| R-free | 0.24818 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cap |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.227 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.700 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.118 | 0.456 |
| Number of reflections | 20314 | |
| <I/σ(I)> | 12.3 | 2.6 |
| Completeness [%] | 98.9 | 99.3 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | AMMONIUM SULFATE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
