3DMH
T. Thermophilus 16S rRNA N2 G1207 methyltransferase (RsmC) in complex with AdoMet and Guanosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9797 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.863, 86.234, 95.091 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.550 |
R-factor | 0.19518 |
Rwork | 0.194 |
R-free | 0.22577 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.361 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.066 | 0.418 |
Number of reflections | 57244 | |
<I/σ(I)> | 23.8 | 2.2 |
Completeness [%] | 97.8 | 81.6 |
Redundancy | 5.7 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | microbatch technique under oil | 8.5 | 277 | 0.085 M TRIS HCl (pH 8.5), 25.5% w/v PEG4000 15% glycerol, microbatch technique under oil, temperature 277K |