3DJU
Crystal structure of human BTG2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.272, 40.751, 67.971 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.940 - 2.260 |
| R-factor | 0.19759 |
| Rwork | 0.194 |
| R-free | 0.26341 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d5r |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.452 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.260 |
| Rmerge | 0.057 |
| Number of reflections | 5309 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 289 | 100mM BIS-TRIS, 0.2M Sodium chloride, 21% (v/v) PEG 3350, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
