3DJN

Crystal structure of mouse TIS21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-05-15
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	38.020, 39.704, 83.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.620 - 2.200
R-factor	0.20228
Rwork	0.196
R-free	0.26162
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d5r
RMSD bond length	0.013
RMSD bond angle	1.474
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Rmerge	0.115
Number of reflections	6868
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	100mM BIS-TRIS, 23% (v/v) PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K