3DJL
Crystal structure of alkylation response protein E. coli AidB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-03-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.02891 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 84.989, 101.875, 137.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.630 - 1.700 |
| R-factor | 0.1618 |
| Rwork | 0.161 |
| R-free | 0.17876 |
| Structure solution method | MIR |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.592 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 62854 | |
| <I/σ(I)> | 80.9 | 8.8 |
| Completeness [%] | 95.7 | 74.8 |
| Redundancy | 12.6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 100 mM Tris pH 8.0, 50 mM CaCl2, and 4% PEG 2000, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
