3DJH
Macrophage Migration Inhibitory Factor (MIF) at 1.25 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.95000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.232, 68.255, 86.512 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.610 - 1.250 |
| R-factor | 0.17018 |
| Rwork | 0.170 |
| R-free | 0.18625 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ce4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.128 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.101 | 0.498 |
| Number of reflections | 110755 | |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 99.0 | 96.9 |
| Redundancy | 4.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 50% Saturated ammonium sulfate, 3% Isopropanol, 0.1 M Tris(hydroxymethyl)aminomethane, mixed with protein in a 1:1 ratio, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
