3DH1
Crystal structure of human tRNA-specific adenosine-34 deaminase subunit ADAT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.28130 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.530, 103.120, 77.590 |
| Unit cell angles | 90.00, 97.60, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.20502 |
| Rwork | 0.202 |
| R-free | 0.25614 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nx8 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.419 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.137 | 0.746 |
| Number of reflections | 18284 | |
| <I/σ(I)> | 12.64 | 3.88 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 7.2 | 6.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25% PEG 3350, 0.2M Ammonium acetate, 0.1M Bis-Tris pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
