3DE8
Crystal Structure of a Dimeric Cytochrome cb562 Assembly Induced by Copper Coordination
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-23 |
| Detector | ADSC Quantum 325 |
| Wavelength(s) | 1.008 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.566, 89.504, 52.019 |
| Unit cell angles | 90.00, 111.01, 90.00 |
Refinement procedure
| Resolution | 32.920 - 1.720 |
| R-factor | 0.19783 |
| Rwork | 0.194 |
| R-free | 0.24378 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.223 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Number of reflections | 42391 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 98.3 | 100 |
| Redundancy | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 mM Hepes, 25% PEG 3350, 200 mM CaCl2, 17.8 mM CuSO4, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
