3DDO

X-RAY Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with by Phosphate Ion at 1.5A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-08
Detector	MAR CCD 165 mm
Wavelength(s)	1.0500
Spacegroup name	P 21 21 21
Unit cell lengths	89.600, 124.920, 134.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.908 - 1.500
R-factor	0.169
Rwork	0.166
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c74
RMSD bond length	0.007
RMSD bond angle	1.094
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.100
High resolution limit [Å]	1.480
Rmerge	0.212
Number of reflections	246188
Completeness [%]	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	298	20% PEG 4000, 0.1M potassium phosphate, 0.2M magnesium chloride, 0.1M cacodylate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K