3DD2
Crystal structure of an RNA aptamer bound to human thrombin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 98 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 83.766, 134.017, 44.033 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.350 - 1.900 |
| Rwork | 0.204 |
| R-free | 0.25400 |
| Starting model (for MR) | 1ppb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 37211 | |
| Completeness [%] | 93.2 | 70 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | PEG 8000, Magnesium Acetate, Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | Magnesium Acetate | ||
| 3 | 1 | 1 | Sodium Cacodylate | ||
| 4 | 1 | 2 | PEG 8000 | ||
| 5 | 1 | 2 | Magnesium Acetate | ||
| 6 | 1 | 2 | Sodium Cacodylate |
