3DCY
Crystal Structure a TP53-induced glycolysis and apoptosis regulator protein from Homo sapiens.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97942 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.983, 76.408, 79.536 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.748 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.22900 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.479 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
High resolution limit [Å] | 1.748 | 3.770 | 1.750 |
Rmerge | 0.109 | 0.068 | 0.540 |
Number of reflections | 25927 | ||
<I/σ(I)> | 16.223 | 2.369 | |
Completeness [%] | 99.8 | 99.9 | 98.4 |
Redundancy | 13.9 | 13.8 | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 297 | PROTEIN SOLUTION (10 MG/ML SEMET PROTEIN, 0.050 M NACL, 0.0003 M TCEP, 0.005 M MES PH 6.0) MIXED IN A 1:1 RATIO WITH WELL SOLUTION (20% PEG 3350, 0.10 M MES PH 6.0) CRYOPROTECTED WITH 20% ETHYLENE GLYCOL, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 297K |