3DCG
Crystal Structure of the HIV Vif BC-box in Complex with Human ElonginB and ElonginC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.514, 66.913, 122.642 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.370 - 2.400 |
| R-factor | 0.18826 |
| Rwork | 0.186 |
| R-free | 0.23228 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vcb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.132 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.370 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.096 | 0.441 |
| Number of reflections | 18219 | |
| <I/σ(I)> | 11.7 | 2.5 |
| Completeness [%] | 98.2 | 97.3 |
| Redundancy | 3.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.1M Tris HCl pH 7.0, 40% PEG 350 MME, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
