3DA5
Crystal Structure of Piwi/Argonaute/Zwille(PAZ) domain from Thermococcus thioreducens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.392, 60.896, 66.447 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.900 - 1.940 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u04 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.772 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.900 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.046 | 0.320 |
| Number of reflections | 14536 | |
| <I/σ(I)> | 30.7 | 3.23 |
| Completeness [%] | 81.1 | 59.4 |
| Redundancy | 4.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | Dioxane, PEG8000, pH 8.5, vapor diffusion, temperature 298K |
