3DA5
Crystal Structure of Piwi/Argonaute/Zwille(PAZ) domain from Thermococcus thioreducens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.392, 60.896, 66.447 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.900 - 1.940 |
R-factor | 0.218 |
Rwork | 0.216 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u04 |
RMSD bond length | 0.021 |
RMSD bond angle | 1.772 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.900 | 2.010 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.046 | 0.320 |
Number of reflections | 14536 | |
<I/σ(I)> | 30.7 | 3.23 |
Completeness [%] | 81.1 | 59.4 |
Redundancy | 4.9 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 298 | Dioxane, PEG8000, pH 8.5, vapor diffusion, temperature 298K |