3D9N
Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the Carboxy-Terminal Domain of RNA-Polymerase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0001 |
| Spacegroup name | P 43 |
| Unit cell lengths | 57.530, 57.530, 106.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.990 - 1.600 |
| R-factor | 0.17348 |
| Rwork | 0.172 |
| R-free | 0.20111 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3d9j |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.269 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC (5.2.0019) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 44673 | |
| <I/σ(I)> | 20.7 | 4.8 |
| Completeness [%] | 98.2 | 94.9 |
| Redundancy | 5.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 3.2 M (NH4)2SO4, 1% (v/v) ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
