3D9K
Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the Carboxy-Terminal Domain of RNA-Polymerase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 43 |
| Unit cell lengths | 56.450, 56.450, 106.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.890 - 2.200 |
| R-factor | 0.19284 |
| Rwork | 0.190 |
| R-free | 0.25161 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3d9j |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.195 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC (5.2.0019) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 16475 | |
| <I/σ(I)> | 18.3 | 5.3 |
| Completeness [%] | 97.3 | 92.6 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.2M Li2SO4, 1.6M (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
