3D9A
High Resolution Crystal Structure Structure of HyHel10 Fab Complexed to Hen Egg Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.842, 77.385, 89.115 |
| Unit cell angles | 90.00, 96.66, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.200 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ndm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.456 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0057) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.230 |
| High resolution limit [Å] | 1.200 | 2.960 | 1.200 |
| Rmerge | 0.039 | 0.027 | 0.580 |
| Number of reflections | 166019 | ||
| <I/σ(I)> | 12.3 | ||
| Completeness [%] | 99.4 | 91.6 | 100 |
| Redundancy | 4.1 | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2M di-Ammonium Citrate; 20% PEG 3350, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
