3D97
Crystal Structure of the R132K:R111L:L121E Mutant of Apo-Cellular Retinoic Acid Binding Protein Type II At 1.50 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-06-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.553, 37.220, 61.058 |
| Unit cell angles | 73.40, 73.70, 89.73 |
Refinement procedure
| Resolution | 55.900 - 1.500 |
| R-factor | 0.161 |
| Rwork | 0.154 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fs6 |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.325 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.900 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.043 | 0.298 |
| Number of reflections | 42526 | |
| <I/σ(I)> | 8.3 | 2.4 |
| Completeness [%] | 96.1 | 93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 30% (w/v) PEG 4000, 0.1 M Bis-TRIS Propane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
