3D8Y
RNase A- 5'-Deoxy-5'-N-piperidinothymidine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.161, 32.421, 72.567 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.720 |
| R-factor | 0.19137 |
| Rwork | 0.189 |
| R-free | 0.22960 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2g8q |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.204 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.720 | 1.700 |
| Number of reflections | 24530 | |
| <I/σ(I)> | 9.2 | 3 |
| Completeness [%] | 97.9 | 96.7 |
| Redundancy | 10.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | PEG 4000, SODIUM CITRATE, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
