3D5K
Crystal structure of the OprM channel in a non-symmetrical space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.900, 70.200, 344.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.400 |
| R-factor | 0.23996 |
| Rwork | 0.236 |
| R-free | 0.29320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wp1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.888 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.710 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 69761 | |
| Completeness [%] | 98.7 | 94.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | Sodium citrate 1M, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
