3D2Z
Complex of the N-acetylmuramyl-L-alanine amidase AmiD from E.coli with the product L-Ala-D-gamma-Glu-L-Lys
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 89.160, 89.160, 183.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.900 - 2.800 |
| R-factor | 0.20526 |
| Rwork | 0.202 |
| R-free | 0.26469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2BGX |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.356 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.263 | 0.698 |
| Number of reflections | 11312 | |
| <I/σ(I)> | 14.6 | 4.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.4 | 21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 1M LICL, 10 % PEG 6K, 0.1M ZNCL2, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
