3D2Y
Complex of the N-acetylmuramyl-L-alanine amidase AmiD from E.coli with the substrate anhydro-N-acetylmuramic acid-L-Ala-D-gamma-Glu-L-Lys
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978850 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 88.274, 88.274, 181.834 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.150 - 1.750 |
| R-factor | 0.20919 |
| Rwork | 0.207 |
| R-free | 0.25326 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2BGX |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.494 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.079 | 0.825 |
| Number of reflections | 42426 | |
| <I/σ(I)> | 28.6 | 2.2 |
| Completeness [%] | 98.6 | 92.7 |
| Redundancy | 17 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 1M LiCl, 10 % PEG 6K, 0.1M ZnCl2, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
