3D2W
Crystal structure of mouse TDP-43 RRM2 domain in complex with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 123 |
Detector technology | CCD |
Collection date | 2008-01-23 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 41.283, 87.290, 125.345 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.460 - 1.650 |
R-factor | 0.21031 |
Rwork | 0.207 |
R-free | 0.24515 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wf0 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.257 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CCP4 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.460 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.058 | 0.320 |
Number of reflections | 13854 | |
<I/σ(I)> | 14 | 3.51 |
Completeness [%] | 98.7 | 97 |
Redundancy | 4.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | Reservoir solution containing 2M (NH4)2SO4, 0.1M phosphate-citrate, the reservoir solution contained an addition of 10% glycerol, pH4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)2SO4 | ||
2 | 1 | 1 | phosphate-citrate | ||
3 | 1 | 1 | glycerol | ||
4 | 1 | 1 | HOH | ||
5 | 1 | 2 | (NH4)2SO4 | ||
6 | 1 | 2 | phosphate-citrate | ||
7 | 1 | 2 | glycerol | ||
8 | 1 | 2 | HOH |