3D29
Proteasome Inhibition by Fellutamide B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2006-04-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 134.272, 301.580, 143.454 |
| Unit cell angles | 90.00, 112.70, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.26600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ryp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.630 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.066 | 0.485 |
| Number of reflections | 336246 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 98.7 | 99.2 |
| Redundancy | 2.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 10 % MPD; 0,1 M MES, 30 mM MgAc2, pH 6,8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
