3D27

E. coli methionine aminopeptidase with Fe inhibitor W29

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	38.510, 59.880, 54.880
Unit cell angles	90.00, 106.47, 90.00

Refinement procedure

Resolution	26.860 - 2.200
R-factor	0.193
Rwork	0.191
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.065
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.880	39.530	2.320
High resolution limit [Å]	2.200	6.960	2.200
Rmerge	0.070	0.052	0.216
Total number of observations		1494	6819
Number of reflections	12282
<I/σ(I)>	8.4	11	3.3
Completeness [%]	100.0	99.7	100
Redundancy	3.9	3.6	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100 mM MES (pH 6.5), 2 mM MnCl2, 17% PEG 20000, vapor diffusion, hanging drop, temperature 298K