3CZD
Crystal structure of human glutaminase in complex with L-glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97891 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 139.520, 139.520, 153.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.000 - 2.400 |
| R-factor | 0.18982 |
| Rwork | 0.189 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2DFW |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.407 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.051 | 0.292 |
| Number of reflections | 29652 | |
| <I/σ(I)> | 26.9 | 7.4 |
| Completeness [%] | 99.0 | 96.7 |
| Redundancy | 9.4 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.4M Lithium sulfate, 100mM Bis-tris-propane pH 7.0, 10mM L-glutamate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
