3CX7

Crystal Structure of PDZRhoGEF rgRGS Domain in a Complex with Galpha-13 Bound to GDP-AlF4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97939
Spacegroup name	P 21 21 21
Unit cell lengths	57.952, 66.371, 151.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.250
R-factor	0.264
Rwork	0.229
R-free	0.26900
Starting model (for MR)	1zcb 1htj
RMSD bond length	0.008
RMSD bond angle	1.323
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.300
High resolution limit [Å]	2.250	2.250
Number of reflections	26672
<I/σ(I)>	25.6	2
Completeness [%]	93.6	60.2
Redundancy	5.9	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	293	18-23 % polyethylene glycol monomethyl ether 2000 and 100 mM Tris, pH 6.9-7.3, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K